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Image Search Results
Journal: International Journal of Molecular Sciences
Article Title: Systematical Analysis of the Protein Targets of Lactoferricin B and Histatin-5 Using Yeast Proteome Microarrays
doi: 10.3390/ijms20174218
Figure Lengend Snippet: The schematic diagram of this study. Yeast proteome microarrays were fabricated from the ~5800 yeast proteins which were individually expressed and purified from yeast. To identify the protein targets, the fabricated yeast proteome microarrays were probed with biotinylated Lfcin B and Histatin-5, individually. Followed by the probing of DyLight 650 labeled streptavidin and DyLight 550 labeled anti-GST antibody on the yeast proteome microarrays to detect the signal of biotinylated AMPs bound to their specific binding partners and the amount of relative protein on proteome microarray, respectively. The identified hits of Lfcin B and Histatin-5 were systematically analyzed for GO and synthetic lethal pairs by using several online bioinformatics databases (DAVID tools and Synthetic Lethality).
Article Snippet: To monitor the shape, size, and uniformity for each protein spot on a chip,
Techniques: Purification, Labeling, Binding Assay, Microarray
Journal: International Journal of Molecular Sciences
Article Title: Systematical Analysis of the Protein Targets of Lactoferricin B and Histatin-5 Using Yeast Proteome Microarrays
doi: 10.3390/ijms20174218
Figure Lengend Snippet: The representative yeast proteome microarray images and the representative hits of Lfcin B and Histatin-5 on yeast proteome microarrays. Images show the microarray and the enlarge hit image of Lfcin B and Histatin-5. Red and green color denotes the signal from DyLight 650 labeled streptavidin and DyLight 550 labeled anti-GST antibody, respectively. Red spots represent the signal of Lfcin B and Histatin-5 on yeast proteome microarrays. ( A ) Triplicate microarray images probed with biotinylated Lfcin B. The position of the representative 5 hits image is shown on the microarray. ( B ) Representative enlarged hits images of Lfcin B. ( C ) Triplicate microarray images probed with biotinylated Histatin-5. ( D ) Representative enlarged hits images of Histatin-5.
Article Snippet: To monitor the shape, size, and uniformity for each protein spot on a chip,
Techniques: Microarray, Labeling